Police have released new CCTV footage in an appeal for witnesses to come forward.
A woman was struck by a large glass bottle, knocking her unconscious and causing dental injuries.
CCTV footage posted by South Yorkshire police shows security escorting a man out of the club, before one of them returns and appears to throw something into the crowd.
The assault occurred on 21 April 2019 at around 10:30pm outside the Honeycomb nightclub on Ecclesall road in Sheffield.
Officers have appealed for witnesses to come forward and give information about the incident.
If you have any information please call 101 quoting incident number 1067 of 21 April 2019.